Fig. 6

Effects of Fars2 deficiency on mitochondria. A?B ATP and ROS levels in the cortex of the cKO mouse embryo compared with control at E 14.5 (mean?±?SD; Two-tailed unpaired t-Test, **P?<?0.01; ****P?<?0.0001; n?=?6 for ATP; n?=?3 for ROS). C?D TP and ROS levels in control and Fars2 knocked-down neurons at the indicated days of culture (mean?±?SD; two-tailed unpaired t-Test, ns: non-significant; **P?<?0.01; ****P?<?0.0001; n?=?4?5 for ATP; n?=?27 for ROS). E Relative mitochondrial membrane potential in the cortex of the cKO mouse embryo compared with control at E 14.5 (mean?±?SD; two-tailed unpaired t-Test, ***P?<?0.001; n?=?3). F Relative NAD_total level in the cortex of the cKO mouse embryo compared with control at E 17.5 (mean?±?SD; two-tailed unpaired t-Test, ns: non-significant; **P?<?0.01; n?=?4). G NAD⁺ and NADH levels of mitochondria pool in control and cKO mice embryo cortex at E 17.5 (mean?±?SD; two-tailed unpaired t-Test, ns: non-significant; **P?<?0.01; n?=?4). H?INAD_total levels and NAD⁺/NADH ratio in control and Fars2 knocked-down neurons at the indicated days of culture (mean?±?SD; two-tailed unpaired t-Test, **P?<?0.01; ***P?<?0.001; ****P?<?0.0001; n?=?5?6). J Transmission electron micrograph of mitochondria in cortex from control and cKO mice embryos at the indicated embryonic ages. Scale bars: 0.5 ?m for E 14.5 and E 17.5 samples and 5 ?m for P 0.5 samples. K Percentage of mitochondria with abnormal cristae in CTRL and cKO cortex at indicated embryonic ages (mean?±?SD; two-tailed unpaired t-Test, *P?<?0.05; n?=?3). L Transmission electron micrograph of mitochondria in control and Fars2 knocked-down neurons at the indicated days of culture. Linearized mitochondrial cristae and the geometrical shapes are indicated by red lines and asterisks. Scale bars: 0.5 ?m. M Percentage of mitochondria with abnormal cristae in control and Fars2 knocked-down neurons at the indicated days of culture (mean?±?SD; two-tailed unpaired t-Test, **P?<?0.01; ***P?<?0.001; n?=?3)