Fig. 5
- ID
- ZDB-FIG-220715-34
- Publication
- Chen et al., 2022 - Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function
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Translation and assembly of OXPHOS subunits are disrupted in Fars2-deficient samples. A?C Western blotting for OXPHOS complexes and the mitochondrial marker TOMM20 in control and cKO mice embryo cortex at indicated embryonic ages (mean?±?SD; two-tailed unpaired t-Test, ns: non-significant; *P?<?0.05; **P?<?0.01; ***P?<?0.001; ****P?<?0.0001; n?=?4). D?F Western blotting for ND1, CO2 and CYTB in control and cKO mice embryo cortex at the indicated embryonic ages (mean?±?SD; two-tailed unpaired t-Test, ns: non-significant; *P?<?0.05; **P?<?0.01; ***P?<?0.001; ****P?<?0.0001; n?=?4). G?H Western blotting for OXPHOS complexes and the mitochondrial marker TOMM20 in control and Fars2 knocked-down neurons at the indicated days of culture (mean?±?SD; two-tailed unpaired t-Test, ns: non-significant; *P?<?0.05; n?=?3). I?J Western blotting for ND1 in control and Fars2 knocked-down neurons at 16 days of infection (mean?±?SD; two-tailed unpaired t-Test, *P?<?0.05; n?=?3). K?L Quantification of TOMM20 in A, B and G (mean?±?SD; two-tailed unpaired t-Test, ns: non-significant; n?=?3?4). M RT-PCR analysis of OXPHOS subunits transcription levels in control and cKO mice embryo cortex at E 17.5 (mean?±?SD; Two-tailed unpaired t-Test, ns: non-significant; *P?<?0.05; **P?<?0.01; n?=?3?4). N qRT-PCR analysis of OXPHOS subunits transcription levels in control and Fars2 knocked-down neurons at 16 days after infection (mean?±?SD; Two-tailed unpaired t-Test, ns: non-significant; *P?<?0.05; **P?<?0.01; n?=?3) |