ZFIN ID: ZDB-FIG-220629-73
Dash et al., 2022 - Nucleolin loss of function leads to aberrant Fibroblast Growth Factor signaling and craniofacial anomalies. Development (Cambridge, England)   149(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 16-cell to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Protruding-mouth

Fig. 1

Ncl expression during zebrafish development. (A) During embryogenesis, Nucleolin (Ncl, red) was ubiquitously expressed in the cytoplasm of four-cell stage wild-type embryo at 1.5 hpf as observed by immunostaining. (B) Similarly, 3 hpf embryos also had ubiquitous cytoplasmic expression of Nucleolin. (C,C′) At 12 hpf, ncl+/+ and ncl−/− embryos exhibited similar Nucleolin expression in the nucleus and cytoplasm in most cells of the embryos. (D-D″) At 18 hpf, the expression of Nucleolin in ncl+/+ embryos was ubiquitous and was confined to the nucleus (D″). In the ncl−/− embryos, the expression pattern of Nucleolin was similar to that of wild-type embryos; however, the expression levels were significantly lower than that of the wild type. (E,E′) By 24 hpf, the expression of Nucleolin was still ubiquitous, with higher levels in the eye and the midbrain-hindbrain boundary in ncl+/+ embryos, whereas it was absent in ncl−/− embryos. (F) At 36 hpf, the expression of Nucleolin became specific to the craniofacial region in the pharyngeal arches as well as the eye. (G) In 72 hpf (3 dpf) wild-type zebrafish, Nucleolin was highly expressed in the jaw of the embryo. (F′,G′) In the ncl−/− mutants, there was no expression of Nucleolin. n=15 for each panel. The experiment was performed three times. NT, neural tube. Scale bars: 35 µm (A,B); 70 µm (C,C′); 140 µm (D,D′); 50 µm (D″); 250 µm (E,E′); 300 µm (F,F′,G,G′).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-ncl IHC AB/TU standard conditions 16-cell whole organism cytoplasm
IHC 1k-cell whole organism cytoplasm
IHC 5-9 somites whole organism cytoplasm
IHC 5-9 somites whole organism nucleus
IHC 14-19 somites whole organism nucleus
IHC Prim-5 whole organism nucleus
IHC Prim-25 eye nucleus
IHC Prim-25 head nucleus
IHC Prim-25 pharyngeal arch nucleus
IHC Protruding-mouth ventral mandibular arch nucleus
IHC nclhi2078Tg/hi2078Tg (AB/TU) standard conditions 5-9 somites whole organism cytoplasm
IHC 5-9 somites whole organism nucleus
IHC 14-19 somites whole organism nucleus
IHC Prim-5 Not Detected whole organism
IHC Prim-25 Not Detected whole organism
IHC Protruding-mouth Not Detected whole organism
Phenotype Details
Fish Conditions Stage Phenotype
nclhi2078Tg/hi2078Tg (AB/TU) standard conditions 14-19 somites whole organism nucleus ab1-ncl labeling decreased distribution, abnormal
Prim-5 whole organism ab1-ncl labeling absent, abnormal
Prim-25 whole organism ab1-ncl labeling absent, abnormal
Protruding-mouth whole organism ab1-ncl labeling absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development