FIGURE

Fig. 5

ID

ZDB-FIG-220629-5

Publication

Morgan et al., 2022 - Pacsin2 is required for endocytosis in the zebrafish pronephric tubule

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Fig. 5

Loss of Pacsin2 results in reduced abundance of the apical endocytic organelles in the proximal tubule. (A) Block-face SEM images of transverse proximal tubule sections from pacsin2^+/− (left panels) and pacsin2^−/− (right panels) 4 dpf larvae. Top panels show whole cross-section of the proximal tubule, bottom panels show enlarged boxed regions indicated in green and red. Top panels: scale bar: 10 µm. Green and red boxed areas=4 µm and 3 µm, respectively. bb, brush border; V, apical vacuolar endosome; N, nucleus; *, lysosomes; arrows, dense apical recycling tubules; arrowheads, endocytic vesicles. (B,C) Average number and size of AVEs, respectively, and (D) number of lysosomes per cell. Unpaired t-test, **** P<0.0001. For B and D, n=40 (pacsin2^+/−) and 34 (pacsin2^−/−). For C, n=114 (pacsin2^+/−) and 136 (pacsin2^−/−). Error bars=s.d.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; pacsin2^umc405/+; mpv17^a9/a9 (AB) mitfa^w2/w2; pacsin2^umc405/+; pacsin2^umc406/+; mpv17^a9/a9 (AB) mitfa^w2/w2; pacsin2^umc406/+; mpv17^a9/a9 (AB)
Observed In:	pronephric proximal convoluted tubule apical recycling endosome pronephric proximal convoluted tubule lysosome
Stage:	Day 4

Phenotype Detail

Acknowledgments

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