FIGURE

Fig. 1

ID

ZDB-FIG-220628-1

Publication

Imani et al., 2022 - Dysregulated myosin in Hermansky-Pudlak syndrome lung fibroblasts is associated with increased cell motility

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Enhanced HPSLF migration in vitro is HPS1-dependent. A, F Representative microscopy images of scratch assays at 0, 12, and 24 h after gaps were generated. Scratch assays were performed with A NLF (n = 3 technical replicates) and HPSLF (n = 3 technical replicates); F NLF transfected with control or HPS1 siRNA; B, G Quantitative analysis of the migration potential of B NLF and HPSLF; G NLF transfected with control or HPS1 siRNA. The rate of fibroblast migration was determined by calculating the percentage of open wound area at the indicated time points to that of the corresponding initial scratch. C Ratio of CFSE MFI from proliferating cells over undivided cells D Western blot analysis of HPS1 protein expression in NLF transfected with either control or HPS1 siRNA. β-Actin was used as a loading control. E Ratio of HPS1 to β-Actin density expressed as fold change relative to NLF transfected with control siRNA. Data are expressed as mean ± SEM of three independent experiments. B, G Data analyzed using two-way mixed ANOVA *P < 0.05. C, E Data was analyzed using Student’s t-test *P < 0.05, **P < 0.01

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Respir Res