FIGURE
Fig. 4
- ID
- ZDB-FIG-220621-4
- Publication
- Yu et al., 2022 - A non-polar fraction of Saponaria officinalis L. acted as a TLR4/MD2 complex antagonist and inhibited TLR4/MyD88 signaling in vitro and in vivo
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Fig. 4
fr.1 reduced the pro-inflammatory effects of LPS in THP-1 macrophages. (A) THP-1 macrophages were incubated for 24 h with sub-fractions at 0–20 μg/ml. Cell viabilities were measured using an EZ-cytox assay kit. Results are expressed as percentages of levels in non-treated THP-1 macrophages. (B) Relative expressions of iNOS and COX-2 were analyzed by western blot. Relative gene expression levels of iNOS and COX-2 as determined by Real-time PCR. (C) Relative expressions of MAPKs as determined by western blot. (D) Relative phosphorylation of IκB-α and total protein levels as determined by western blot. The nuclear translocation of NF-κB (p65) was determined by western blot. Results are presented as the means ± SDs of three different experiments. ##p < .01 versus non-treated THP-1 macrophages, and *p < .05, **p < .01 versus LPS-treated THP-1 macrophages.
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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