FIGURE

Fig. 9

ID

ZDB-FIG-220607-28

Publication

Li et al., 2022 - Claudin-5a is essential for the functional formation of both zebrafish blood-brain barrier and blood-cerebrospinal fluid barrier

Other Figures

All Figure Page

Back to All Figure Page

Fig. 9

Maintenance of CP epithelial polarity and junctions in cldn5a^-/- larvae. A Apical polarity marker of PKCζ and epithelial junction proteins were stained to investigate the integrity of dCP epithelium. In the larval brains of wild types and cldn5a^-/- with NM, PKCζ colocalizes with Cldn5a and is linearly expressed in the apical side of dCP epithelium. In cldn5a^-/- with BE, PKCζ localizes non-apically and misexpresses on the protruded CP into the ventricle (white arrowhead) and the misfolded plexus epithelial layers (white arrow). Furthermore, compared to that in wild types, cldn5a^-/- larvae show a dramatic upregulation of ZO-1. There was no discrepancy in the expression of Beta-catenin between wild types and cldn5a^-/- mutated larvae. White dot lines figure out the linear dCP epithelium. n > 3 fishes analyzed per group. Scale bars: 20 μm. B, C The quantitative analysis of ZO-1 and Beta-catenin by measuring the mean gray values in CP epithelial cells with ImageJ software. Data are shown as mean ± SEM. ***P < 0.005, ****P < 0.001

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Fluids Barriers CNS