The whiB4::Tn mutant is defective in resuscitation from hypoxia-induced dormancy. (A) Growth of WT, whiB4::Tn, and the complemented strain (COM) under hypoxia. Data (mean ± SD) are from three biological replicates. There was no significant difference on the growth curves as analyzed by two-way analysis of variance (ANOVA). (B) Growth of hypoxic cultures of WT, whiB4::Tn, and the complemented strain (COM) after reaeration. Data (mean ± SD) are from three biological replicates. The CFU of whiB4::Tn was significantly lower than that of WT or the complemented strain at 48 and 72 h postaeration. *, P < 0.05; ****, P < 0.0001, two-way ANOVA. (C) Overlaps of DEGs in whiB4::Tn and the complemented strain (COM). Genes that were downregulated in whiB4::Tn relative to WT overlapped significantly with genes that were upregulated in the complemented strain relative to WT at 24 and 48 h postaeration. (D) Heatmap of the mRNA levels of the DosR regulon and sigE. The mRNA level of each gene was normalized against the value of the same gene at 0 h time point (7-day hypoxic cultures) and plotted as log2.
|
