Figure 6

Figure 6. RT-PCR: (A) ascl1a was significantly expressed in the early stages of regeneration beginning at 2 dpi through to 7 dpi. (B) Significant expression of lin28a from 1 to 7 dpi. (C) wnt4a showed significant upregulation at 100 dpi. (D) stat3 showed no differential expression throughout any of the time points, 1–200 dpi. (E,F) mmp9 showed no changes in expression, mmp2 was downregulated throughout all time points. No expression was detected at time points 1 dpi, 2 dpi, 5 dpi, 7 dpi, 10 dpi and 20 dpi for both, mmp9 and mmp2. (G) tgfbi showed significant change in expression at 7 dpi. (H) cxcr5 showed differential expression at 1–30 dpi. No expression was detected at time points 1 dpi, 2 dpi, 5 dpi, 7 dpi, 10 dpi and 20 dpi. (I) gata3 reported no significant differential expression at any of the time points. No expression was detected at time points 1 dpi, 2 dpi, 5 dpi, 7 dpi, 10 dpi and 20 dpi. Uninjured retina—0 dpi. β-actin served as reference gene. Three independent replicates were carried out for each gene at each time point. Error bars represent +/− standard error of the mean (SEM). Stars used to denote significance * p value < 0.01, ** p value < 0.005, **** p value < 0.0001.