FIGURE

Fig. 1

ID

ZDB-FIG-220415-25

Publication

Banono et al., 2020 - Zebrafish Larvae Carrying a Splice Variant Mutation in cacna1d: A New Model for Schizophrenia-Like Behaviours?

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Fig. 1

Zebrafish cacna1da mutant allele (sa17298) and its molecular consequence. a Schematic representation of the zebrafish cacna1da transcript. Red bars represent exons and lines represent introns. Image retrieved from ensembl.org. b Schematic representation of zebrafish cacna1da and sa17298 mutation. Nucleotides in upper case are within the exon while those in lower case are within the intron. c Sample gel electrophoresis of BstEII restriction digest and resulting PCR products to determine genotype of fish/larvae. BstEII does not cut homozygous sa17298, but cuts WT into two bands (70 and 119 bp) and heterozygous sa17298 into three bands (70, 119 and 196 bp). Note the multiple bands in the BstEII digested versus the undigested corresponding samples. L: 1 kilobase DNA ladder, +/+: cacna1da^WT/WT, +/−: cacna1da^sa17298/WT, −/−: cacna1da^{sa17298/sa17298}.dcacna1da mRNA levels as measured by agarose gel electrophoresis (rt-PCR). ecacna1da mRNA levels as measured by qPCR. cacna1da mRNA expression is normalised against gapdh and rps18

Expression Data

Gene:	cacna1da
Fish:	WT cacna1da^{sa17298/sa17298} cacna1da^sa17298/+
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cacna1da^{sa17298/sa17298} cacna1da^sa17298/+
Observed In:	whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurobiol.