p38 signaling pathway is involved in dusp14 regulation of inner ear hearing in zebrafish. (A) The imaging analysis of control and dusp14 mutants at 72 hpf in bright field and fluorescent field. (A′) Quantification of the number of the unilateral hair cell clusters of control and dusp14 mutants at 72 hpf. (B) Confocal imaging analysis of inner ear hair cells and lateral line neuromast hair cells of control, dusp14 deficiency and dusp14-MO and p38 inhibitor coinjected zebrafish at 72 hpf. (B′) The statistical analysis of the numbers of crista hair cells for panel (B). (B′′) The statistical analysis of the numbers of hair cells in the remaining neuromast L3 for panel (B). (C) The representation of SOX2 immunofluorescence images of neuromasts in the posterior lateral line of the control, dusp14 morphants and dusp14-MO and p38 inhibitor coinjected zebrafish at 72 hpf. (C′) Quantification of the number of supporting cells per neuromast for panel (C). (D) BrdU staining for the supporting cells in the neuromasts of the control, dusp14 morphants and dusp14-MO and p38 inhibitor coinjected zebrafish at 72 hpf. (D′) Quantification of zebrafish embryos with the BrdU+ cells for panel (D). Experimental embryos were sampled at 72 hpf (n > 6). Each bar represents the mean ± SD. Values with ** and **** above the bars are significantly different (P < 0.01 and P < 0.0001, respectively).
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