Figure 3

MITF mutation prevents PPP6C-mediated melanocyte differentiation disruption. (A) Schematic of the human MITF-M and zebrafish Mitfa proteins. (B) Representative images of 5 day old fish on the roy;nacre background injected with either MC-GFP;mitfa(S8D,S12D), MC-PPP6C;mitfa(S8D,S12D), or MC-PPP6C(R264C);mitfa(S8D,S12D). (C) Representative counts of melanocytes of 5 day old fish on the roy;nacre background injected with either MC-GFP, MC-PPP6C, or MC-PPP6C(R264C) and with either MITF(S8,S12) or the MITF(S8D, S12D) mutations (Student’s two-tailed t-test). (D) Real-time qPCR measurement of SOX10 and MITF in 48 h post fertilization fish injected with either MC-GFP, MC-PPP6C or MC-PPP6C(R264C) and with either MITF(S8,S12) or the MITF(S8D, S12D) mutations (Student’s two-tailed t-test). (E) Real-time qPCR measurement of DCT, TYR, and TYRP1B in 48 h post fertilization fish injected with either MC-GFP, MC-PPP6C or MC-PPP6C(R264C) with and with either MITF(S8,S12) or the MITF(S8D, S12D) mutations. All data panels in the figure are representative of at least three experiments. p-values are indicated as following: *p < 0.05, **p ≤ 0.01, ****p ≤ 0.0001.