FIGURE 1

Phylogenetic analysis, conservative sequence, and protein structure prediction of BMP7 genes. (A) Phylogenetic analysis of BMP7 genes among different species. (B) The structural analysis of BMP7 genes among different species. The green triangle represents the knockout site of bmp7a and bmp7b. (C) Conservation analysis of amino acids in BMP7 genes’ domains among different species. (D) Schematic representation of bmp7a and bmp7b DNA and protein sequences, and the mutant alleles for bmp7a and bmp7b. CRISPR-Cas9 target sequence is marked with red letters in the wild and mutant DNA sequences. The missing nucleotides are represented by (-) in the mutant, and the red box represents the stop codon. (E) The bmp7a protein tertiary structure prediction of wild type and mutation with -19 bp deletion. The yellow area represents the α-helix; the green area represents the β-sheet; the red area represents the missing part of the mutation. (F) The bmp7b protein tertiary structure prediction of wild type and mutation with -49 bp deletion. The yellow area represents the α-helix; the green area represents the β-sheet; the red area represents the missing part of the mutation.