FIGURE

Figure S1.

ID

ZDB-FIG-220328-13

Publication

Krishnan et al., 2022 - Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo

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Figure S1.

Differences in mitotic centrosome movement towards the cytokinetic bridge during pre-abscission between zebrafish embryos and human cells.

(A, B) Overlay of centrosome movement within pre-abscising zebrafish embryo cell (A) and human (HeLa) cell (B) over time. The centrosome is labeled with centrin-GFP (zebrafish embryo cell, gray) and DsRed-PACT (human HeLa cell, gray). Pink dashed line with arrow, centrosome track. Orange dashed lines, cell boundaries. Scale bar, 10 μm. (C) Time-lapse of a pre-abscising human (HeLa) cell expressing centrin-GFP (inverted grays). Green dot, centroid of cell. Orange dashed lines, cell boundaries. Brown line, width of the cytokinetic bridge. Cyan line, distance of the centrosome from the midpoint of the cytokinetic bridge (μm). Scale bar, 10 μm. (D) Model depicting quantification related to centrosome movement towards the cytokinetic bridge. Magenta circle, centrosomes. Black line, cell boundaries. Brown line, width of the cytokinetic bridge. Cyan line, distance of the centrosome from the midpoint of cytokinetic bridge (μm). (E) Percentage of pre-abscising cells (%) that moves the centrosome to at least 2, 4, 6, and 8 μm from the midway point of the cytokinetic bridge.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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