FIGURE

Fig 6

ID

ZDB-FIG-220325-24

Publication

Lu et al., 2022 - mTOR activity is essential for retinal pigment epithelium regeneration in zebrafish

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Fig 6

Inhibition of mTOR activity impaired macrophage/microglia recruitment to the RPE layer post-ablation.

(A) Schematic of the experimental paradigm showing the timeline for chemical treatments and ablation on transgenic larvae (mpeg1:mCherry;rpe65a:nfsB-eGFP). (B-E) Immunofluorescent images of mCherry staining on transverse cryosections from MTZ^- and MTZ⁺ DMSO- and rapamycin-treated larvae at 3dpi. Nuclei (white), mCherry (magenta). (F) Quantification of the mCherry signal in the RPE layer showed a significant increase in MTZ⁺ DMSO-treated larvae, when compared to MTZ^- DMSO controls. The percent of the RPE covered by mCherry⁺ cells was significantly reduced in MTZ⁺ rapamycin-treated larvae when compared to MTZ⁺ DMSO-treated controls. However, there was no significant difference between MTZ^- DMSO-treated and rapamycin-treated groups. p-values: * ≤ 0.05, ** ≤ 0.01. Statistical information can be found in S9 Table. Dorsal is up and distal is left. Scale bar = 50μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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