Intranasal SARS-CoV-2 S RBD protein delivery causes widespread losses of olfactory receptor expression in adult zebrafish. (A-B) Bar plots showing fold-change in expression of olfactory receptor genes in the zebrafish OO 3 h and 3 d post intranasal SARS-CoV-2 S RBD protein compared to vehicle controls identified by scRNA-Seq. (C-D) Violin plots showing changes in significantly differentially expressed ompb expression, a marker of mature ciliated OSNs, in zebrafish OO cell suspensions, across all olfactory neuronal cell clusters in 3 h SARS-CoV-2 S RBD (orange) and 3 d SARS-CoV-2 S RBD (green) versus vehicle treated (blue). (E-F) Summary webs of gene ontology analyses performed in ShinyGO v0.61 from the scRNA-Seq data showing the number of significantly up and down regulated genes across olfactory neuronal clusters in zebrafish OO 3 h and 3 d post-intranasal SARS-CoV-2 S RBD protein versus vehicle treated controls. (G-H) Gene set enrichment analysis using Metascape of genes from all neuronal clusters that were either significantly up or down regulated in zebrafish OO 3 h and 3 d post-intranasal SARS-CoV-2 S RBD protein compared to vehicle treated controls (adjusted P-value< 0.05). Enriched terms are colored by P-value.
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