FIGURE

Figure 4—figure supplement 4.

ID

ZDB-FIG-220314-82

Publication

Qi et al., 2022 - Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish

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Figure 4—figure supplement 4.

Notch signaling represses endocardial protrusion formation.

(A–D) Confocal projection images of the heart of Tg(myl7:mCherry-CAAX); Tg(tp1-MmHbb:EGFP) (A–B) and Tg(cdh5:Gal4ff); Tg(UAS:LIFEACT-GFP) (C–D) embryos at 48 hours post-fertilization (hpf). (A–B) Treatment with 1 µM of the Notch inhibitor RO4929097 from 24 to 48 hpf blocks the expression of the Tg(tp1-MmHbb:EGFP) Notch reporter in the endocardium. (C–D) Embryos treated with the Notch inhibitor exhibit fewer endocardial touchdowns (asterisks). (E–F) Embryos treated with the Notch inhibitor exhibit more endocardial protrusions (arrows). (G) Quantification of the number of endocardial touchdowns in the ventricle of DMSO and Notch inhibitor treated embryos at 48 hpf. (H) Quantification of the number of endocardial protrusions in the ventricle of DMSO and RO4929097 treated embryos at 48 hpf. All images are ventral views, anterior to the top. V, ventricle; A, atrium. Data in graphs expressed as mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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