Figure 6

Thioredoxin affects neutrophil motility around KRas^G12V-transformed keratinocytes. (A) Neutrophil (blue) recruitment around KRas^G12V-expressing basal keratinocytes (magenta) in txn^+/+ Tg(LyzC:H2B-mcherry) or txn^-/- Tg(LyzC:H2B-mcherry) larvae 3dpf. (B) Quantification of neutrophil abundance in txn wt (n=74) and mutant (n=116) larvae. (C) Stills from live imaging of neutrophil behavior around KRas^G12V-expressing cells in txn wt and mutant larvae. Neutrophils were tracked across frames (1.5min/frame) and track color delineates instantaneous neutrophil velocity. (D) Quantification of average neutrophil speed in txn wt (n=4) and mutant (n=4) larvae. (E) TRAP-qPCR of KRas^G12V-expressing genes assessing inflammatory cytokines il1b, cxcl8a, cxcl8b.1, tnfa (pool, 30 larvae/replicate, wt n=3, mut n=3). Scale bar, 10uM. Bolded shapes indicate average values per larvae, and small shapes represent instantaneous velocities of individual neutrophils. Samples were analyzed for statistical significance via Mann-Whitney U test (B) and t-test (D, E) (p < 0.01**). ns, not significant.