Figure 3

Thioredoxin regulates neutrophil recruitment and redox balance in damaged tissues. (A) Schematic of txn gene with annotations of gRNA target site and 1bp insertion identified in txn mutant larvae. (B) Western blot of Txn and αTubulin on whole lysates from txn wildtype and mutant larvae. (C) Schematic of tail transection assay to measure neutrophil recruitment and H₂O₂ abundance conducted on txn^+/+ Tg(LyzC:H2B-mcherry) or txn^-/-Tg(LyzC:H2B-mcherry) larvae 3dpf. (D) pfbs-f probe for H₂O₂ (1uM) in response to tail transection 30mpw (wt n=56, mut n=54) and 4hpw (wt n=51, mut n=51) with (E) quantification of signal density in region posterior to notochord. (F) Neutrophil recruitment in response to tail transection 2hpw (wt n=79, mut n=85) and 6hpw (wt n=87, mut n=90) with (G) quantification of neutrophil abundance in region posterior to notochord. Scale bar, 50um. Large, bolded shapes indicate average value per replicate (n), with small shapes representing data points from independent larvae. Samples were analyzed for statistical significance by Mann-Whitney U test (p < 0.001***, p < 0.0001****). ns, not significant.