Fig. 4

Survival rate, sublethal and teratogenic abnormalities of zebrafish after depletion of the natterin-like gene by the CRISPR/Cas9 system. The depletion of the natterin-like gene LOC795232 in 0 h post-fertilization (hpf) zebrafish embryos was done by the synthetic duplex guide RNA (tracrRNA + natterin-like crRNA, 50 μg.nL^− 1) with Cas9 (250 μg.nL^− 1). The survival of the depleted larvae (KO) and wild-type (WT) group (A), sublethality (B), and teratogenicity (C) endpoints were analyzed at 24, 48, 72, 96, 120, and 144 hpf (20 embryos per group). The dashed line indicates the percentage of mortality acceptable by the OECD of 30%. The lines indicate the average of abnormalities in the KO group at each time measured. The asterisks (*) represent a significant difference with the WT (p < 0.05), which is represented in the complementary graph in the bottom of B and C. See additional file 2 for raw data of 20 embryos per group behind the figures