FIGURE

Fig. 4

ID

ZDB-FIG-220107-19

Publication

Bertho et al., 2021 - A transgenic system for targeted ablation of reproductive and maternal-effect genes

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Fig. 4

(A) Schematic depicting the site targeted in buc and representative mutant alleles. (B-C?) F1 progeny of F0 female transgenic for GMS:buc are (B,B?) polarized or (C,C?) lack polarity. (D) Representative T7 endonuclease assays to detect buc mutations. Mutagenesis frequencies for F1 progeny of two founder males. (E-H) Bright-field images and (E?-H?) maternal GFP expression in (E,E?) progeny of an OMS:buc F1 female and (F-H?) progeny of OMS:buc F0 female. (F,F?) GFP? embryo with normal polarity. (G,G?) GFP+ embryo with normal polarity. (H,H?) GFP+ apolar embryo. (I) Table shows transmission frequency and phenotype penetrance of GMS:buc F1 progeny.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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