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Fig. 4 (A) Schematic depicting the site targeted in buc and representative mutant alleles. (B-C?) F1 progeny of F0 female transgenic for GMS:buc are (B,B?) polarized or (C,C?) lack polarity. (D) Representative T7 endonuclease assays to detect buc mutations. Mutagenesis frequencies for F1 progeny of two founder males. (E-H) Bright-field images and (E?-H?) maternal GFP expression in (E,E?) progeny of an OMS:buc F1 female and (F-H?) progeny of OMS:buc F0 female. (F,F?) GFP? embryo with normal polarity. (G,G?) GFP+ embryo with normal polarity. (H,H?) GFP+ apolar embryo. (I) Table shows transmission frequency and phenotype penetrance of GMS:buc F1 progeny.