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Immunohistochemical staining of Abcb4 and Abcb5 with the C219 antibody in adult zebrafish. Whole adult zebrafish were stained with the C219 antibody (a) or negative control (b), as noted in Materials and Methods. Bar = 5 mm for (a) and (b). Regions of interest are indicated (a) and expanded in subsequent panels (c–h). C219 staining of the zebrafish brain (c) with an enlarged portion of the cerebellum (d). Positive staining of C219 antibody was also noted in gill epithelium (e), a subset of renal tubules or collecting ducts (*) (f), hepatocytes (g), and the epidermis (h). Bar = 700 µm for (c) and 200 µm for (d–h).
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