Fig. 7.

Differential light exposure in pcdh15b mutants attenuates or exacerbates photoreceptor defects. (A) Representative TEM images of wild-type sibling and pcdh15b mutant ONL at 10 dpf grown under 0 lux (dark) and 6000 lux (bright) light conditions. The dotted line in the top image separates the inner and outer row. ISs with detached OSs are coloured magenta. Deformed OSs are coloured green. (B-F) Quantification of the percentage of total OSs found in the inner row (B), the percentage of deformed OSs in the outer row (C) and the inner row (D), the average OS length (E), and the percentage of total identified photoreceptor IS/cell bodies with completely detached/missing OSs (F) across the retina photoreceptor layer under different light conditions in wild-type siblings and pcdh15b Δ7 mutants. All graphs in B-F use the same labelling as in B to distinguish between +/+ and Δ7/Δ7. x-axis labels are the same and seen at the bottom in E and F. (G) Representative phalloidin staining in the ONL, labelling CPs, at 10 dpf when exposed to 3000 lux bright light (+/+, 7 eyes; Δ7/Δ7, 7 eyes). 0 lux measurements were taken across the entire ONL, and 6000 lux measurements were taken only in the central ONL. Statistical analyses in B-D and F were performed using two-way ANOVA. Statistical tests in E were performed using one-way ANOVA. n.s., not significant; *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001. Numbers of eyes/OSs analysed: 0 lux: +/+, 6 eyes/1239 OSs; Δ7/Δ7, 8 eyes/1623 OSs; 6000 lux: +/+, 7 eyes/541 OSs; Δ7/Δ7, 6 eyes/409 OSs. IS, inner segment; OS, outer segment.