FIGURE

Figure 3

ID

ZDB-FIG-211216-148

Publication

Dalum et al., 2021 - High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue

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Figure 3

The four zebrafish gill arches display the same lymphoid organization. Representative deconvolved confocal images of an adult zebrafish branchial cavity showing the 4 gills arches with transversal orientation (A). The plane of section is illustrated by the scheme at the bottom left. The images were acquired from 30 μm whole-body cryosections stained with phalloidin (green) and DAPI (blue) and where T/NK cells were labeled with anti-ZAP70 antibody (red hot). Each gill arch (Ga1-Ga4) possesses a GIALT segmented in five sub-regions (1-5) (ILT, interlamellar region-lamellae-efferent aspect of filaments, interbranchial septum, gill arch, T/NK cell clusters at the base of filaments on each side of the gill arch). (B) Schematic representation of (A) displaying the 5 sub-regions of the GIALT. (A) The image is a maximum intensity projection of 15 µm. Annotations: Bc, Branchial cavity; Ga, Gill arch and S, Septum. Scale bar: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol