FIGURE

Figure 2

ID

ZDB-FIG-211129-80

Publication

Bhatia et al., 2021 - Quantitative spatial and temporal assessment of regulatory element activity in zebrafish

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Figure 2

Characterisation of SHH-SBE2 landing line.

(A) Top: schematic of the design of the landing pad bearing SHH-SBE2 as the tracking cis-regulatory element (CRE). Below: CRE activity observed exclusively in the forebrain in F1 embryos with the SHH-SBE2-eGFP tracking cassette. Images shown for pool of F1 embryos (scale bar = 1000 ?m) and individual embryos (scale bar = 100 ?m) at different stages of embryonic development. FB, forebrain; hpf, hr post fertilisation. Scale bar = 100 ?m. (B) Unique bands (*) observed in round 2 of PCR amplification of DpnI digested genomic DNA from F1 embryos bearing the landing pad cassette. (C) Ensembl Genome Browser snapshot depicting the integration site (red arrow) of the SHH-SBE2 landing pad and sequencing data from clones bearing the PCR product shown by * in (B).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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