FIGURE

Figure 6

ID

ZDB-FIG-211129-70

Publication

Su et al., 2021 - Xylose-configured cyclophellitols as selective inhibitors for glucocerebrosidase

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Figure 6

In vivo inhibitory effect of β‐d‐xylo‐configured compounds on β‐glucosidases in zebrafish (Danio rerio) larvae. (A) Larvae were exposed to 5 dpf with the indicated inhibitor 1 or 2 in the medium. Larvae were lysed and incubated with fluorescent ABP 10. Fluorescently labeled proteins were visualized after SDS‐PAGE, only GBA and GBA2 were assessed in zebrafish larvae model. (B) Apparent IC₅₀ values towards β‐glucosidases (GBA and GBA2) were determined by the fluorescence quantification based on cABPP results. (C) In vivo inhibition curves. (D) GlcSph levels in zebrafish larvae were determined as described in experiment section, n=2 replicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL
Condition:	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor
Observed In:	whole organism D-glucosylsphingosine
Stage:	Day 5

Phenotype Detail

Acknowledgments

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