FIGURE

Figure 5

ID

ZDB-FIG-211129-69

Publication

Su et al., 2021 - Xylose-configured cyclophellitols as selective inhibitors for glucocerebrosidase

Other Figures

All Figure Page

Back to All Figure Page

Figure 5

Inhibitory effect of β‐d‐xylo‐configured cyclophellitol 1 and cyclophellitol aziridine 2 on β‐glucosidases in intact HEK293T cells expressing GBA, GBA2 and GBA3. (A) Representative gel images of cABPP where cells were treated for 24 h with indicated inhibitor. Lysates were then prepared and labeled with fluorescent ABP 10. Fluorescently labeled proteins were visualized after SDS‐PAGE (1 set from n=3 replicates). (B) IC₅₀ curves determined by cABPP labeling results. (C) Apparent IC₅₀ values towards β‐glucosidases in intact HEK293T cells producing GBA, GBA2 and GBA3 were determined by the fluorescence quantification based on cABPP SDS‐PAGE results.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Chembiochem