Fig. 5

Evaluation of the role of overexpression of miR-26a on anti-inflammatory activity of TnP. Lateral view of representative zebrafish larvae demonstrated strong expression of miR-26a in the head of both groups WT LPS/TnP and Mimic LPS/TnP, analyzed by miRCURY LNA miRNA digoxigenin-labeled detection probe. Dotted red circle around the head indicates positive chromogenic detection (A). Embryos were visualized on AxioVision® software (Carl Zeiss, Oberkochen, Germany) in magnification of 100×. (B) Neutrophilia to wound tail was assessed after TnP treatment of LPS-stimulated mimic larvae. WT-Control with amputated caudal fins non-stimulated and non-treated was considered as the control. At the end of experiments, groups of larvae were fixed and stained with Sudan black solution for the absolute neutrophil number count in 500 μm from the cut of the tail. Data represent mean ± SEM and *p < 0.01 compared with WT-Control group, #p < 0.01 compared with WT LPS-group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)