Fig 3

Quantification of neutrophil dispersion out of CHT of wild-type and Spint1a-deficient embryos treated with 100 μM NAC (A), 100 μM mito-TEMPO (MT) and 100 nM tempol (T) (C), 250 μM apocynin (G), or upon genetic inactivation of nox1 and nox5 (I, J) and nox4 (I, M) with CRISPR/Cas-9. Representative merge images (brightfield and red channels) of lyz:dsRED zebrafish larvae of every group are shown (B, D, F, H, K, M). Determination of NFKB transcriptional activity in the skin of embryos treated with MT and T (E) and representative images (green channel) of nfkb:eGFP zebrafish larvae of every group (F). Each dot represents one individual, and the mean ± SEM for each group is also shown. p-Values were calculated using 1-way ANOVA and Tukey multiple range test and t test. ns, not significant, **p ≤ 0.01, ****p ≤ 0.0001. The data underlying this figure can be found in S1 Data. ANOVA, analysis of variance; CHT, caudal hematopoietic tissue; NAC, N-acetylcysteine; ROS, reactive oxygen species.