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Fig. 5

ID

ZDB-FIG-211105-18

Publication

Eom et al., 2021 - Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes

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Fig. 5

Jam3b promotes aggregation in vitro and an adherent morphology in vivo. (A) S2 cells transfected with constructs to express actin-GFP (control), wild-type Jam3b-mCherry or A96E mutant Jam3b-mCherry. Aggregates of cells expressing wild-type Jam3b formed within 60 min of rotary culture. (B) Quantification of transfected cells found within clusters relative to the total numbers of transfected cells in 3 replicate cultures. ∗∗∗, Tukey-Kramer post-hoc comparisons of means P < 0.0001 (overall ANOVA, F2,6 = 82.6, P < 0.0001; data were arcsine-transformed for analysis to correct heterogeneity of variance among groups). (C) Membrane-targeted mCherry revealed well-spread morphologies of wild-type melanophores but more dendritic morphologies of jam3butr8e1 and igsf11^utr15e1 mutant melanophores in vivo. (D) Melanophores of jam3b^vp8re2 mutants were also dendritic but adopted a more spread appearance when expressing wild-type Jam3b transgenically. Scale bars 20 μM (D, for C and D).

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 476, Eom, D.S., Patterson, L.B., Bostic, R.R., Parichy, D.M., Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes, 314-327, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.