FIGURE

Fig. 3

ID

ZDB-FIG-211103-92

Publication

Ye et al., 2021 - Identification of in vivo Hox13-binding sites reveals an essential locus controlling zebrafish brachyury expression

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Hoxa13b-binding sites and motif analysis. (A) Distribution of the Hoxa13b target sites in the genome. Most sites are in distal intergenic regions. (B) The Hoxa13b-binding motif discovered from analysis of the CUT&RUN data. The red arrowhead indicates a base that, as a T, is bound by all Hox proteins, whereas, as a C, it is bound by posterior Hox proteins, based on in vitro studies. (C) The Hoxa13b motif shown in B is centrally located among all the CUT&RUN DNA fragments that contain a Hox motif, as expected. (D) Hoxa13b forms a 19?bp footprint at the Hoxa13b motif sites in the HS:hoxa13b-FLAG-GFP sample (teal), whereas, in the control sample, DNA cutting by MNase was random and rare at the same sites (red).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development