FIGURE

Figure 5.

ID

ZDB-FIG-211103-75

Publication

Holmgren et al., 2021 - Mechanical overstimulation causes acute injury and synapse loss followed by fast recovery in lateral-line neuromasts of larval zebrafish

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Figure 5.

Mechanically overstimulated neuromasts showed retracted neurites and detached synaptic debris.

(A–B) Representative images of control (A) and exposed (B) neuromasts. Synaptic ribbons (magenta; Ribeye b) and PSDs (green; MAGUK) were immunolabeled; hair cells were also immunolabeled, but not shown for clarity. Afferent neurons (white) were labeled with GFP. Insets: Arrows indicate intact synapses adjacent to afferent neurons; arrowheads (B) indicate synaptic debris. Scale bars: 5 µm (main panels), 1 µm (insets). (C) Frequency histogram of observed synaptic debris per neuromast (NM). While control neuromasts occasionally had one detached synapse, exposed neuromasts were observed that had up to five detached synapses.

Expression Data

Antibodies:	Ab1-pan-MAGUK Ab3-ctbp2l
Fish:	AB
Conditions:	mechanical stress ablation: lateral line, chemical treatment by environment: copper(II) sulfate, mechanical stress
Anatomical Terms:	neuromast hair cell postsynaptic density neuromast hair cell synaptic ribbon
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	mechanical stress ablation: lateral line, chemical treatment by environment: copper(II) sulfate, mechanical stress
Observed In:	neuromast hair cell postsynaptic density neuromast hair cell synaptic ribbon
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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