Figure 6
- ID
- ZDB-FIG-211103-42
- Publication
- Lim et al., 2021 - The LAMMER Kinase, LkhA, Affects Aspergillus fumigatus Pathogenicity by Modulating Reproduction and Biosynthesis of Cell Wall PAMPs
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Role of AflkhA in biosynthesis of conidial cell walls. Expression level of ?-1,3-glucan synthase fks1 in vegetative cells (A) and conidia (B). Spores were inoculated in MM liquid culture and incubated for the indicated number of hrs. Total RNA was extracted and RT-qPCR analysis was performed using 18S rRNA gene as an internal control. **P < 0.01, ***P < 0.001. (C) Amount of ?-glucan (pg) per 103 conidia in WT and ?AflkhA strains (n = 4). **P < 0.01 (D) Concentration (ng/mL) of GM. Conidia was inoculated in modified Brian medium and cultured at 37°C for 24 hrs. Extracellular GM content in the culture supernatant was assayed by ELISA (n = 4). *P < 0.05. |