FIGURE

Figure 2

ID

ZDB-FIG-211103-31

Publication

Kler et al., 2021 - Cre-Dependent Anterograde Transsynaptic Labeling and Functional Imaging in Zebrafish Using VSV With Reduced Cytotoxicity

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Figure 2

Cre-dependent TRAS-M51R labeling of specific retinorecipient neuron subtypes. (A–A”) Representative image of a 2 dpi (5 dpf) live vglut2a:LRL-GFP larvae injected with VSV(M51R)ΔG-Cre. Boxed area in (A) is shown in higher magnification in (A’; both show maximum z-projection). Panel (A”) shows a single optical section in the box area in (A), at the level of AF9. GFP expression was seen in the injected eye (arrowhead in A), retinorecipient cells (round green cells), and the AFs (AF9 and AF10 shown in A”). (B–B”) Representative image of a 2 dpi (5 dpf) live gad1b:LRL-GFP larvae injected with VSV(M51R)ΔG-Cre. Boxed area in (B) is shown in higher magnification in (B’) (both show maximum z-projection). Panel (B”) shows a single optical section in the box area in (B), at the level of AF9. GFP expression was seen in the injected eye (arrowhead in B) and retinorecipient cells. No AF labeling was seen. Green fluorescence in the uninjected eye was from the autofluorescence of retinal pigment epithelial cells. Scale bars are 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.