FIGURE

Figure 4

ID

ZDB-FIG-211029-87

Publication

Athapaththu et al., 2021 - Gamma-Aminobutyric Acid (GABA) Promotes Growth in Zebrafish Larvae by Inducing IGF-1 Expression via GABA_A and GABA_B Receptors

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Figure 4

GABA upregulates growth-stimulating gene expression via GABA_A and GABA_B receptors. Zebrafish larvae (n = 20) at three days post-fertilization (dpf) were treated with 10 �M of each (A) bicuculline, (B) CGP 46381, and (C) TPMPA 2 h before treatment with 25 mM GABA, and total RNA was extracted at 9 dpf. The RNA was reverse-transcribed, and synthetic cDNA was amplified to determine expression of the genes for insulin-like growth factor 1 (zIGF-1), growth hormone 1 (zGH-1), growth hormone receptor 1 (zGHR-1), and cholecystokinin A (zCCKA). z?-Actin was used as an internal control. Densitometry analysis was conducted to determine the expression level of each gene relative to that of ?-actin and illustrated (bottom). Significant differences among the groups were determined using Student?s t-test. All data are presented as mean � standard error of the mean (*** p < 0.001). N.S., non-significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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