FIGURE

Fig 1

ID

ZDB-FIG-211025-4

Publication

Mall et al., 2021 - Heading towards a dead end: The role of DND1 in germ line differentiation of human iPSCs

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Fig 1

(A) End-point PCR showing presence of large deletions in DND1 genomic locus. (B) All lines exhibited similar doubling times of about 20 hours. Doubling times counted from 4 independent passages per line. (C) About 90% of TS hiPSCs were double positive for OCT4 and NANOG, irrespective of DND1 status. CB hiPSCs showed reduced NANOG expression with 52–81% double positive cells. (D) RT-qPCR showed similar expression of OCT4, SOX2, and DNMTB1 in all lines compared to H9 hESCs. CB hiPSCs showed about 50% reduction of NANOG expression, irrespective of DND1 status. DND1 is expressed in low levels in hESCs and DND1+/+ hiPSCs and mRNA levels are strongly reduced in DND1-/- hiPSCs. The data were normalised to two internal controls (ACTB and GAPDH) and are shown as fold change to H9 ESCs. N = 3 biological replicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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