Apoptosis and adhesion assay. (a) Apoptotic assay demonstrated that CRB2 knockout t17 and t32 clones were more susceptible to apoptosis under serum starvation than wild-type human podocytes. (b) CRB2 knockout t17 clone was more susceptible to apoptosis under high glucose conditions than wild-type human podocytes, while there was no significant difference between CRB2 knockout t32 clone and wild-type podocytes. Data are presented as mean ± SD. Statistical analysis by one-way analysis of variance, followed by Scheffe's multiple comparison test; n = 4 in each group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (c) Adhesion assay showed decreased adhesive ability in CRB2 knockout t32 clone compared to wild-type podocytes or CRB2 knockout t17 clone while there was no significant difference between CRB2 knockout t17 and wild-type podocytes. Data are presented as mean ± SD. Statistical analysis by one-way analysis of variance, followed by Scheffe's multiple comparison test; n = 5 in each group. *P < 0.05, **P < 0.01. CRB2KO CRB2 knockout, FBS fetal bovine serum, HG high glucose, LG low glucose, WT wild-type.
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