FIGURE

Fig. 3.

ID
ZDB-FIG-211018-96
Publication
Iqbal et al., 2020 - Alpha-synuclein aggresomes inhibit ciliogenesis and multiple functions of the centrosome
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3.

Aggresomes reduce rate of cell migration and inhibit polarity changes. (A–D) RPE1 cells close a scratch/wound in 8 h. (E–H) In the presence of aggresomes, minimal cell migration is detected. (I-L) MEFs close a scratch-wound assay in 8 h whereas those treated with MG132 to induce aggresomes fail to do so (M–P). (Q,R) In control cells, the Golgi (red) orientates from a random direction to face the leading edge of the wound. (S,T) In the presence of aggresomes, this change in orientation was not seen. (U,V) Quantification of change in angle of orientation during cell migration with a schematic diagram showing how the Golgi orientation was measured. (P=0.0011, by Student's t-test, 100 cells, n=3 replicates). Scale bars: 100 μM. Nuclei stained with DAPI (blue).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open