Fig. 3.

Fig. 3.

Aggresomes reduce rate of cell migration and inhibit polarity changes. (A–D) RPE1 cells close a scratch/wound in 8 h. (E–H) In the presence of aggresomes, minimal cell migration is detected. (I-L) MEFs close a scratch-wound assay in 8 h whereas those treated with MG132 to induce aggresomes fail to do so (M–P). (Q,R) In control cells, the Golgi (red) orientates from a random direction to face the leading edge of the wound. (S,T) In the presence of aggresomes, this change in orientation was not seen. (U,V) Quantification of change in angle of orientation during cell migration with a schematic diagram showing how the Golgi orientation was measured. (P=0.0011, by Student's t-test, 100 cells, n=3 replicates). Scale bars: 100 μM. Nuclei stained with DAPI (blue).