FIGURE

FIGURE 2

ID

ZDB-FIG-211013-2

Publication

Pakdaman et al., 2021 - Chip Protein U-Box Domain Truncation Affects Purkinje Neuron Morphology and Leads to Behavioral Changes in Zebrafish

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FIGURE 2

Figure 2. stub1 mRNAs are expressed in different tissues and in the Purkinje cells of adult zebrafish cerebellum. (A) Scatter plot of ddPCR data presenting the absolute number of stub1 transcripts per ng of cDNA for different zebrafish tissues. Each dot represents a biological replicate. Means and standard deviations are indicated by the lines and bars in the graph (n = 4). (B) DIG-labeled stub1 cDNA probes were synthesized, and used for visualization of stub1 mRNA expression in adult zebrafish brain sections, using in situ hybridization assay. (C) Detailed view of cerebellum where stub1 positive cells in the Purkinje cell layer are indicated by arrowheads. (D) Negative control corresponding to the same area as shown in (B). Tel, telencephalon; TeO, tectum opticum; TL, torus longitudinalis; Val, valvula cerebelli; Cce, Corpus cerebelli; EG, eminentia granularis; Mo, medulla oblongata; Hyp, hypothalamus; A, anterior; P, posterior; D, dorsal; V, ventral; ML, molecular layer; PCL, Purkinje cell layer; GL, granular layer.

Expression Data

Gene:	stub1
Fish:	AB/TU
Anatomical Terms:	brain cerebellum corpus cerebelli granular eminence granular layer corpus cerebelli head kidney heart hypothalamus integument intestine liver medulla oblongata midbrain muscle oocyte Purkinje cell layer corpus cerebelli telencephalon testis thyroid follicle torus longitudinalis valvula cerebelli
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.