FIGURE

Fig. 1

ID

ZDB-FIG-211008-1

Publication

Ross et al., 2021 - Generation and Molecular Characterization of Transient tet1/2/3 Zebrafish Knockouts

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Fig. 1

Phenotypes of tet1/2/3 KO F0 zebrafish following CRISPR/Cas9 microinjections. (a) Zebrafish Tet protein structure and guide RNA design. (b) Top left: uninjected 24 hpf zebrafish, bottom left: tet1/2/3 KO injected 24 hpf zebrafish displaying improperly formed midbrain hindbrain boundary (i) and microphthalmia (ii). Top right: uninjected 48 hpf zebrafish; bottom right: tet1/2/3 KO injected 48 hpf zebrafish displaying microphthalmia (iii), altered pigmentation and curved trunk (iv). (c) qPCR quantification of two biological replicates of uninjected wild type (wt) and injected (tet1/2/3 KO) F0 zebrafish. Each gene is tested in triplicate and represented as the mean relative expression to wt. Error bars represent standard deviation

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Meth. Mol. Biol.