FIGURE

Figure 5

ID

ZDB-FIG-210927-18

Publication

Kim et al., 2021 - A zebrafish screen reveals Renin-angiotensin system inhibitors as neuroprotective via mitochondrial restoration in dopamine neurons

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Figure 5

DA neuron-specific RNA-seq uncovers neurotoxic insult-induced alterations of mitochondrial pathway gene expression that is in part restored by the AGTR1 inhibitor olmesartan.

(A) A schematic showing the RNA-seq procedure of larval samples from chemical treatment to FACs, library preparation, and differential gene expression analysis. (B) A heatmap of clustering analysis comparing the differential gene expression in DMSO control, olmesartan, CBE, MTZ, MTZ+ olmesartan, and CBE+ olmesartan treatment groups. Gene counts were normalized and analyzed with the R program DESeq2 package. All samples are numbered 1, 2, and 3 to indicate biological replicates. (C–D) Venn diagrams showing the overlapping gene expression alterations between different conditions: MTZ/control and CBE/control (C) and MTZ+ Olmesartan/MTZ and CBE+ Olmesartan/CBE (D) (α = 0.05, FDR = 0.1, Wald test). (E–F) Metascape ontology clusters highlighting the top enriched GO terms for differential gene expression common between that of MTZ/Control and CBE/Control (E) and that of MTZ+ olmesartan/MTZ and CBE+ olmesartan/CBE (F). The colors of the nodes correspond to significant values. The size of the nodes is proportional to the number of input genes in the GO term. The most significant GO terms in both (E) and (F) include oxidative phosphorylation, respiratory electron transport, ATP metabolic process, and inorganic cation transport.

Expression Data

Gene:	atp6ap2
Fish:	th^{ion32Tg/ion32Tg}; c264Tg/c264Tg
Conditions:	chemical treatment by environment: conduritol B epoxide chemical treatment by environment: metronidazole
Anatomical Term:	forebrain dopaminergic neuron
Stage Range:	Day 5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	th^{ion32Tg/ion32Tg}; c264Tg/c264Tg
Conditions:	chemical treatment by environment: L-dopa chemical treatment by environment: conduritol B epoxide chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole
Observed In:	dopaminergic neuron ATP metabolic process dopaminergic neuron inorganic cation transmembrane transport dopaminergic neuron inorganic ion transmembrane transport dopaminergic neuron oxidative phosphorylation dopaminergic neuron respiratory electron transport chain dopaminergic neuron ribonucleotide biosynthetic process forebrain dopaminergic neuron
Stage Range:	Day 5 to Day 6

Phenotype Detail

Acknowledgments

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