FIGURE

Fig. 4

ID

ZDB-FIG-210925-4

Publication

Linsley et al., 2021 - Genetically encoded cell-death indicators (GEDI) to detect an early irreversible commitment to neurodegeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Comparison of GEDI variants to detect neuronal death.

A–C Representative images of rat primary cortical neurons expressing RGEDI-P2a-3xBFP (A), mRuby-P2a-GCaMP6f (B), or RGEDI-P2a-EGFP (C), before, 5 min and 10 min after exposure to NaN₃. D Quantification of the peak signal and response rate of signal increase (τ) from fitted nonlinear regressions of increases in fluorescence signals over time from variants of the GEDI biosensor: RGEDI-P2a-3xBFP (n = 23), mRuby-P2a-GCaMP6f (n = 52), RGEDI-P2a-EGFP (n = 41), GC150-P2a-mApple, RGEDI-NLS-P2a-EGFP-NLS, and GC150-NLS-P2a-mApple-NLS (n = 40), compared to EGFP-P2a-mApple (n = 18). Error bars represent SE, ANOVA Tukey’s ****p < 0.0001; ns not significant, n values represent individual neurons sampled across at least three independent wells. E Relative fluorescence of GECI’s GCaMP6f⁷⁹ and RCEPIA³⁶/RGEDI and GCaMP150ER³⁷/GC150 across Ca²⁺ concentrations modeled from previously reported Hill coefficients and K_d values. F Representative images of rat primary cortical neurons expressing GC150-P2a-mApple, G GC150-NLS-P2a-mApple-NLS, and H RGEDI-NLS-P2a-EGFP-NLS before, 5 min, and 10 min after exposure to NaN₃. Error bars represent SEM. Scale = 25 μm. Experiments in A–C and F–H were repeated at least 18 times with similar results.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.