Gh-transgenic zebrafish (Danio rerio). The F0104 line was developed from the co-injection of two separate transgenes, both containing the carp β-actin promoter (Cyprinus carpio), which controls the expression of the GFP gene from the jellyfish Aequorea victoria or the GH gene from the marine silverside fish (Odonthestes argentinensis). Linearized transgenes were co-injected in an equimolar ratio into zebrafish zygotes to provide the same genome integration and expression (Figueiredo et al., 2007). In the F0104 line, GFP expression works not only as a marker of transgenesis but also allows mapping the tissues that are expressing GH since both GFP and GH are under the control of the same gene promoter.
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