Fig. 3

Immunohistochemistry demonstrating location of Col11a1a within developing craniofacial region at 72 hpf. Wildtype zebrafish embryos were fixed, embedded, and sectioned for immunofluorescence detection of Col11a1a using the new primary antibody. A Schematic representation of the craniofacial region of zebrafish. B Negative control using pre-immune serum in place of primary antibody. C Negative control omitting primary antibody. D Col11a1a primary antibody (green) detects Col11a1a in the eye and within the jaw cartilage elements represented by the mandible (ma) and Meckel’s cartilage (mc). DAPI (blue) staining indicates the location of cell nuclei. Dotted square frames indicate location of higher magnification images of mandibular cartilage shown in E and Meckel’s cartilage shown in F. Scale bars B 50 µm; C 50 µm; D 50 µm; E 250 µm; F 250 µm. Results are representative of staining pattern found in 15 individual embryos