Figure 4

elovl8a and elovl8b gene deletion in zebrafish and effects of elovl8a and elovl8b deletion on liver fatty acid compositions. (A,B) The targeting site for elovl8a (A) and elovl8b (B) gene knockout. The gray boxes were the 5′-untranslated region and 3′-untranslated region and black boxes were the exons. The red box indicated the targeting sequences. (C) The PUFA composition in liver of wild-type (WT) and elovl8a^−/−. (D) The ratio of C20:2n-6/C18:2n-6, C20:3n-3/C18:3n-3, and C22:5n-3/C20:5n-3 in the liver of WT and elovl8a^−/−. (E) The SFA and MUFA composition in the liver of WT and elovl8b^−/−. (F) The ratio of C20:0/C18:0 in the liver of WT and elovl8b^−/−. The statistical analyses were conducted by the t test. Data were expressed as mean ± SD (standard deviation) of four biological replicates. Asterisks above the error bars indicate significant differences (* p < 0.05. aa, amino acid; elovl, elongation of very long-chain fatty acid protein; SFAs, saturated fatty acids; MUFAs, monounsaturated fatty acids; PUFAs, polyunsaturated fatty acids.