PUBLICATION
Evolution and Functional Characteristics of the Novel elovl8 That Play Pivotal Roles in Fatty Acid Biosynthesis
- Authors
- Sun, S., Wang, Y., Goh, P.T., Lopes-Marques, M., Castro, L.F.C., Monroig, Ó., Kuah, M.K., Cao, X., Shu-Chien, A.C., Gao, J.
- ID
- ZDB-PUB-210828-18
- Date
- 2021
- Source
- Genes 12(8): (Journal)
- Registered Authors
- Keywords
- elovl8, fatty acid synthesis, gene knockout, zebrafish
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems/genetics
- Cloning, Molecular
- Evolution, Molecular*
- Fatty Acid Elongases/genetics*
- Fatty Acids/biosynthesis
- Fatty Acids/genetics*
- Fatty Acids, Unsaturated/genetics
- Fatty Acids, Unsaturated/metabolism
- Fish Proteins/genetics*
- Gene Expression Regulation, Enzymologic/genetics
- Lipogenesis/genetics
- Zebrafish/genetics
- PubMed
- 34440461 Full text @ Genes (Basel)
Citation
Sun, S., Wang, Y., Goh, P.T., Lopes-Marques, M., Castro, L.F.C., Monroig, Ó., Kuah, M.K., Cao, X., Shu-Chien, A.C., Gao, J. (2021) Evolution and Functional Characteristics of the Novel elovl8 That Play Pivotal Roles in Fatty Acid Biosynthesis. Genes. 12(8):.
Abstract
Elongation of very long-chain fatty acid (Elovl) proteins are key enzymes that catalyze the rate-limiting step in the fatty acid elongation pathway. The most recently discovered member of the Elovl family, Elovl8, has been proposed to be a fish-specific elongase with two gene paralogs described in teleosts. However, the biological functions of Elovl8 are still to be elucidated. In this study, we showed that in contrast to previous findings, elovl8 is not unique to teleosts, but displays a rather unique and ample phylogenetic distribution. For functional determination, we generated elovl8a (elovl8a-/-) and elovl8b (elovl8b-/-) zebrafish using CRISPR/Cas9 technology. Fatty acid composition in vivo and zebrafish liver cell experiments suggest that the substrate preference of Elovl8 overlapped with other existing Elovl enzymes. Zebrafish Elovl8a could elongate the polyunsaturated fatty acids (PUFAs) C18:2n-6 and C18:3n-3 to C20:2n-6 and C20:3n-3, respectively. Along with PUFA, zebrafish Elovl8b also showed the capacity to elongate C18:0 and C20:1. Gene expression quantification suggests that Elovl8a and Elovl8b may play a potentially important role in fatty acid biosynthesis. Overall, our results provide novel insights into the function of Elovl8a and Elovl8b, representing additional fatty acid elongases not previously described in chordates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping