Figure 8

Effect of HS-binding peptide on tumor progression. Approximately 150 PDX cells obtained by surgical resection of a patient with triple-negative breast cancer were labeled with red fluorescent protein (RFP) and injected into the zebrafish embryo yolk sac after 1 day of fertilization (1 dpf). Embryos were incubated for 18 hours, 35.5°C. Peptide, animals were treated with 10 µM HS-binding peptide. Control, animals were not treated. Green; green fluorescent protein (GFP) labeled blood vessels. Red; red fluorescent protein (RFP) labeled tumor cells. (A) Representative images of the control group and the treated group. (B) Quantification of tumor fluorescence (tumor size). The bar represent the average of red fluorescence in zebrafish embryos. (C) Values express percent survival of animals; red line (10 μM of peptide treatment); blue line (animals in the absence of treatment). Each group contains 10 zebrafish embryos. Peptide decreased the number of tumoral cells and increased survival.