Fig 2

(A-H?) Maximum projections of confocal live imaging Z-stacks acquired from ventral side of wild-type zebrafish head at 3 dpf (A, A?), 5 dpf (B, B?), 7dpf (C, C?), 14 dpf (D, D?) and nkx3.2^{uu2803/uu2803} zebrafish head at 3 dpf (E, E?), 5 dpf (F, F?), 7 dpf (G, G?), 14 dpf (H, H?) in Tg(sox10:egfp) background. Corresponding single Z-planes are shown in A??H?. (A-D´´) The jaw joint in wild-type zebrafish (dashed box) is magnified in A?-D?. The retroarticular process is visible from 3 dpf onwards, marked by white arrowhead. Chondrocytes of the anterior Meckel?s cartilage and posterior palatoquadrate align in stacks. Posterior Meckel?s cartilage and anterior palatoquadrate articulate the jaw joint. (E-H?) Fusion of jaw joint articulating elements in nkx3.2 mutants. The fusion site is magnified in (E?-H?) and indicated by asterisks. (E?-F? and E?-F?) At 3 dpf and 5 dpf nkx3.2 mutants display unorganised and rounded cells in the anterior palatoquadrate, posterior to the jaw joint fusion site (G?, G?) At 7 dpf nkx3.2 mutants display elongated chondrocytes that start to align in stacks. (H?, H?) At 14 dpf nkx3.2 mutants display elongated chondrocytes that are aligned throughout the fused Meckel?s-palatoquadrate element. m?Meckel?s cartilage, pq?palatoquadrate. Scale bars: 100 ?m (A-H), 25 ?m in (A?-H? and A?-H?).